The particle number (PN) emission of diesel engine was measured by using MEXA-2000SPCS. The effects of engine speed, load, EGR rate and load loading on PN emission were studied without post-treatment catalytic converter and the effects of DOC, CDPF carbon load and ambient temperature on PN emission were studied with post-treatment catalytic converter. The results show that PN emission will increase with the increase of speed, will first decrease and then increase with the increase of load, will also increase with the increase of EGR rate due to the in-cylinder combustion deterioration, and will have a rapid and large increase with load loading before using post-treatment catalytic converter. After using post-treatment catalytic converter, DOC can effectively reduce particle mass (PM), but cannot reduce particle number (PN). CDPF carbon load is an important factor of determining PN emission and will reduce PN emission on the premise of non-triggering active regeneration. The passive regeneration rate of CDPF is affected by the ambient temperature and PN emission will become less because the cumulative capacity of CDPF carbon load for the same period becomes stronger due to the decrease of ambient temperature.